Microbial evaluation of fresh, minimally-processed vegetables and bagged sprouts from chain supermarkets.

ABSTRACT The aim of this study was to evaluate the bacterial and fungal quality of minimally-processed vegetables (MPV) and sprouts. A total of 116 samples of fresh-cut vegetables, ready-to-eat salads, and mung bean and wheat sprouts were randomly collected and analyzed. The load of aerobic mesophilic bacteria was minimum and maximum in the fresh-cut vegetables and fresh mung bean sprouts respectively, corresponding to populations of 5.3 and 8.5 log CFU/g. E. coli O157:H7 was found to be absent in all samples; however, other E. coli strains were detected in 21 samples (18.1%), and Salmonella spp. were found in one mung bean (3.1%) and one ready-to-eat salad sample (5%). Yeasts were the predominant organisms and were found in 100% of the samples. Geotrichum, Fusarium, and Penicillium spp. were the most prevalent molds in mung sprouts while Cladosporium and Penicillium spp. were most frequently found in ready-to-eat salad samples. According to results from the present study, effective control measures should be implemented to minimize the microbiological contamination of fresh produce sold in Tehran, Iran.


INTRODUCTION
In many parts of the world, including Iran, there is an increasing rate of consumption of raw fresh produce, like vegetables, fruits, and sprouts. This is especially the case for minimally-processed fruits and vegetables, mainly because of changes in the human lifestyle and their tendency towards convenience and spending less time on preparing food (1)(2)(3). However, despite their nutritional and healthy characteristics, outbreaks of human infections associated with the consumption of fresh or

Microbial Evaluation of Fresh, Minimally-processed Vegetables and Bagged Sprouts from Chain Supermarkets
intact produce and release nutrients and facilitate growth of microorganisms (1,11,12).
Outbreaks of foodborne diseases linked to the consumption of fresh produce have shown a remarkable increase in the last two decades (13). The Center for Science, in the public interest, recently released a report based on the data from the Centers for Disease Control and Prevention (CDC) and other sources, indicating that green leafy vegetables were associated with 363 outbreaks, including 13,569 announced cases of illness (14). An outbreak of E. coli O157:H7 occurred in 26 US states in September 2006, which led to about 200 cases of illness, including some with haemolytic-uraemic syndrome (HUS) and resulted in three deaths (15). Data demonstrated that fresh spinach grown in three Californian counties was responsible for contamination.
Salmonella is the leading cause of foodborne diseases throughout the world. In the last few years, outbreaks of Salmonella have been linked increasingly to consumption of fresh vegetables. This pathogen is the main challenge for the microbiological safety of MPV (16).
Microbiological surveys of MPV products have investigated the occurrence of Salmonella, Escherichia coli, coliforms, total aerobic and spoilage bacteria, fungi, and yeasts (1,(17)(18)(19)(20)(21)(22). Most reported counts for total aerobic bacteria ranged between 4 and 8 log CFU/g and between 0.7 and 6 log CFU/g for coliforms. E. coli strains have been often observed at low prevalence and low counts. Pathogens, like E. coli O157:H7, Salmonella, and L. monocytogenes, have rarely been found.
To the best of our knowledge, there is no published data on the microbiological quality of this fresh produce in Iran. Therefore, this study represents the first survey in microbial contamination in MPV and sprouts. The aim of this study was to investigate the microbiological quality (both bacterial and fungal contamination) in MPV and sprouts, which are commercially available in Iran, aiming at the future improvement of food safety measures.

Collection of samples
A total of 116 samples of MPV and bagged sprouts were randomly collected from four chain supermarkets during May and July 2012 in Tehran city, Iran. Vegetables and sprouts comprised 20 samples of ready-to-eat salads (containing three to five ingredients, such as lettuce, coleslaw, cucumber, car-rots, and tomato), 64 sprout samples (mung and wheat), and 32 samples of fresh-cut vegetables (including seven types of vegetables: Leek, fennel, watercress, basil, and radish). All of the samples were obtained in the original packages before their best-before date, within the shelf-life of up to seven days, as mentioned on the labels. All samples were placed in secure sterile re-sealable plastic bags and transported promptly to the laboratory in ice boxes. Samples that had been unpackaged or visibly damaged were discarded before analysis.

Bacteriological analysis
Manufacturer's name, type of vegetables, batch number, expiry date, and the type of packaging (modified or non-modified atmosphere) were recorded. The surfaces of the packaging were sterilized before samples were taken out of them. This was done using ethanol-sterile gauze to prevent cross-contamination.
Ten grammes of each sample was weighed and placed in a stomacher bag, and it was diluted in 90 mL of buffered peptone water (BPW) and homogenized for 2 min at 260 rev per minute, using a stomacher (Model 400 circulator, Seward, Norfolk, England). Eight decimal dilutions of the suspension were made in BPW and analyzed for aerobic mesophilic count, E. coli, coliforms, molds, and yeasts.
For determination of aerobic mesophilic bacteria, 1 mL of each decimal dilution was added to 12-15 mL plate count agar (PCA), the plates were incubated for 24-48 h in 30 °C, and then the bacterial colonies were counted (23).
To detect Salmonella spp., 25 g of each sample was diluted in 225 mL of BPW and homogenized as previously described. Then, these were held for 16-24 h at 35 °C; 0.1 mL of this sample was added to two broth cultures (Selenite cysteine and Tetrathionate broth) that contained iodine and novobiocin, then was incubated at 35 °C and 41.5 °C for 24 h respectively. For isolation and identification, streaking cultures from two mentioned cultures were done by three differential cultures, including brilliant green bile broth (BGBB), xylose lysine deoxycholate agar (XLD agar) and Salmonella-Shigella agar (SSA) and incubated for 24-48 h at 37 °C; 1-2 colony(ies) were picked up from each positive plate and cultured on three media (Lysine decarboxylase agar triple, sugar iron agar, and urea broth) to be confirmed (24).
For determining the coliforms, 1 mL of each decimal dilution and 15 mL solid culture medium of violet red bile agar (VRBA) were poured to petridish; after stirring, these were held to fix. Then a thin layer of media were poured to make micro-aerophilic condition, and plates were incubated for 24 h at 35 °C; in the end, red colonies were counted (25). Also, presumptive E. coli was determined. At first, 1 mL of primary dilution was added to lauryl sulphate media and incubated at 37 °C for 24 h; if the gas was observed, loopfuls of suspension were transferred to EC broth and kept at 44 °C for 48 h to examine for gas production; gas-positive samples were transferred to PBW to keep at 44 °C for 48 h; indol production was confirmed by using indol reagent, which resulted in red colour that verified presence of E. coli. Differentiation of E. coli was carried out with IMViC test. E. coli strains isolated were plated in Tergitol BCIG agar and Sorbitol-MacConkey agar and incubated at 44±1 °C for the detection of β-glucuronidase and sorbitol-positive strains respectively (26).
For determination of yeasts and molds, 1 mL of each decimal dilution was placed on plate surface that contained Sabouraud dextrose agar (SDA) and distributed by a sterilized swab. Plates were incubated for 5 days at 25 °C. Colonies were counted and expressed as CFU/g; molds were purified on SDA for further subculture for microscopic examination and identification (27).

Statistical analysis
All of the samples were tested in triplicate. Statistical analyses were estimated using SPSS (version 11.5). In order to analyze the data, standard deviation and mean were calculated. Independent t-test were used for determining any statistically significant difference (p<0.05) among two brands of each commodity. Table 1 presents the contamination of freshcut vegetables, ready-to-eat salads, and wheat and mung sprouts to aerobic mesophilic bacteria; the average loads were 6.4±0.7, 6.7±0.5, 6.9±0.6, and 7±0.6 log CFU/g respectively.

Coliforms, E. coli, and Salmonella spp.
The number of total coliforms and pathogens in the vegetables and sprouts are presented in Table  2 and 3. As presented in Table 2, total coliforms were not detected in 13% of the fresh-cut vegetable samples while the rest of the samples were positive. Mung bean sprout samples contained the highest values of thermotolerant coliforms with a mean of 62.5%.  Ready-to-eat salads a Results are expressed as mean±SD of three repetitions and the counts are given in terms of log CFU/g of product; b Range in log CFU/g of products; c Not detected E. coli was detected in 3 out of 32 fresh-cut vegetable samples (9.4%), in 6 out of 20 ready-to-eat salad samples (30%), and in 12 of 64 sprout samples (18.7%) ( Table 3).
Salmonella spp. were not detected in fresh-cut vegetables and wheat sprouts but it was isolated from 1 out of 20 ready-to-eat salad samples (5%) and 1 out of 32 mung bean sprout samples (3.1%) ( Table 3).

Yeasts and molds
The results of mold and yeast counts are presented in Table 4. Yeasts were the predominant organisms and found in 100% of the samples. Molds and yeasts in fresh-cut vegetables, ready-to-eat salads, and sprout samples were in the range of 5.4-7.6, 6.2-7.5, and 6.0-8.5 log CFU/g respectively. Yeast populations were more than 10 6 CFU/g in the entire ready-to-eat salads and sprout samples. In comparison, fresh-cut vegetables generally contained less amounts of yeasts, with 78% of the samples having counts of <10 6 CFU/g ( Table 4).
Majority of the samples showed molds contamination levels between 10 2 and 10 4 CFU/g. The most frequent molds observed in sprout samples comprised Fusarium, Penicillium, and Geotrichum spp., which were found in 25%, 15.6%, and 10.9% of the samples respectively (Table 5). Among the commodities, wheat sprouts (Geotrichum and Fusarium spp.), mung sprouts (Geotrichum and Penicillium spp.), and ready-to eat vegetables (Cladosporium and Penicillium spp.) constituted the most prevalent molds, and ready-to-eat salads generally contained greater values of molds than fresh-cut vegetable samples. Cladosporium, Penicillium, Alternaria, and Geotrichum spp. were the most common filamentous fungi in ready-to-eat salad samples and were present in 35%, 20%, 15%, and 15% respectively.

DISCUSSION
Microbial quality of fresh, minimally-processed vegetables and bagged sprouts obtained from chain supermarkets in Tehran city of Iran was determined. In this study, significant differences were not observed among commercial brands. With respect to the national standards of Iran (28), only 3% of the sprout samples (mung sprout and wheat germ), 28.1% of the fresh-cut vegetable samples, and 5% of the ready-to-eat salad samples, which contained less than 6.0 log CFU/g aerobic mesophilic bacteria, can be considered safe for consumption. The   (29) who reported that the mean count of aerobic mesophilic (6.5 log CFU/g) ranged from 5.8 to 7.3 log CFU/g, in 13 packs of salad. Similarly, Valentin-Bon et al. (20) conducted a study under the supervision of Food and Drug Administration (FDA) in the United States (USA) on 100 bagged lettuce and spinach mixes and revealed that the average count of aerobic mesophilic bacteria was 7 log CFU/g, ranging from <4 to 8.3 log CFU/g. Abadias et al. (1) reported that the average count of aerobic mesophilic bacteria in 236 samples of fresh-cut vegetables was in the range of 4.3 to 8.9 log CFU/g. In another study, the microbial quality of fresh-cut vegetables and fruits was evaluated by Gómez-Govea et al. (2). The results indicated that the levels of mesophilic organisms ranged from 10 to 10 7 CFU/g.
As mentioned on the labels of sprouts and fresh-cut vegetables, it is recommended to wash these products before consumption. Our analysis showed that the maximum reduction of 0.5 log CFU/g in the microbial load is achieved after this washing step, which was not remarkable (data not shown). Because salads are directly used, appropriate and effective washing could not play a substantial role in decreasing the microbial load and caused a maximum of 2 log CFU/g reduction in the fresh-cut vegetables (30).
Due to the favorauble conditions present in sprout samples, including high humidity, high temperatures, and suitable pH and nutrients, microbial population can proliferate fast, especially during sprouting (29,31). Martínez-Villaluenga et al. (31) evaluated the role of germination in the microbial loads of broccoli seeds and reported that the amount of aerobic mesophilic bacteria, total and thermotolerant coliforms increased approximately by 2 and 3 log CFU/g after five days of germination respectively. Hence, these products are exposed to higher pathogenic contamination, such as E. coli and Salmonella spp. compared to vegetables. Therefore, preventive technical approaches should be implemented to ensure safety of sprouts (32).
de Oliveira et al. (21) assessed the microbial quality of 162 MPV samples and detected E. coli in 53.1% of the samples, Salmonella spp. in 1.2% of the samples, and total and thermotolerant coliforms in 81.5% and 66% of the samples respectively (21). Seow et al. (29) mentioned that the average load of coliforms in 13 ready-to-eat salad samples and 14 bean sprout samples were 5.2 and 5.7 log CFU/g respectively, and all samples contained populations greater than 4 log CFU/g. Aycicek et al. (7) pointed out that there are some factors influencing high load of coliform in leafy vegetables, including large surfaces exposed to contamination, intense use of untreated manure during preharvest, and more handling steps during postharvest. In the present study, the population of total coliforms in 95% of the samples exceeded 2 log CFU/g (national standard). In spite of the absence of E. coli O157:H7 in the samples of this study, E. coli strains were detected in 9.4% of fresh- Aspergillus 100-2.4×10 3 5 cut vegetable samples, in 30% of ready-to-eat salad samples, and in 18.7% of sprout samples. Also, E. coli O157:H7 has not been isolated in several studies (1,2,18,22,29). The prevalence of E. coli found in the present study was lower than that in the study by Prado et al. (33), that reported E. coli contamination in 30% of the MPV samples. Based on a report by Abadias et al. (1), E. coli contamination was found in 7.1% of the whole vegetable samples and 11.4% of the fresh-cut vegetable samples; only two freshcut vegetable samples (0.8%) had E. coli counts exceeding 100 MPN/g. In another study (34) on faecal indicator in fresh produce, E. coli strains were identified in 8.1% of carrots, lettuce, green onions, and spinach samples.
In several similar studies on the microbial quality of vegetables, E. coli and Salmonella spp. were either not detected in any of the samples, or these were detected in only one sample (2,22,29,35).  (29). Enterobacter, Erwinia spp. and other non-faecal coliforms bacteria have long been recognized as common organisms in fresh produce, such as lettuce and fresh-cut salads (38). However, their presence is not considered a public health threat but may result in spoilage of vegetables and fruits, such as Erwinia spp., which produce pectolytic enzymes and soften vegetable tissues. Therefore, level of faecal organisms, such as E. coli, is a better indicator of quality of fresh produce, and this could explain why this organism has been included as a hygienic criterion in the new EU regulation (1).
In contrast to the present study, Badosa et al. (39) reported yeast and mold counts in most of the vegetable samples, ranging from 4 to 7 log CFU/g. Acevedo et al. (40) also detected molds in the levels of 4.5×10 4 CFU/g in salad samples. They reported the frequent presence of Penicillium, Aspergillus, and Fusarium spp. in salads. Although organisms, such as Rhizopus stolonifer and Paecilomyces, were found at low levels in sprout samples, these can grow fast and cause spoilage in short time. In addition to the differences in local sanitary conditions from farm to the market, various methodologies used in these studies could yield different results.
Many of the isolated molds belonged to toxigenic genera, such as Penicillium, Alternaria, and Fusarium spp. The high numbers of Penicillium spp. in bean sprout samples and their ability to grow at refrigeration temperatures indicate a potential for mycotoxin production of these foodstuff during marketing (19). According to some authors, the high density of mycotoxin-producing molds generally correspond to poor cleaning practices and/or use of unhygienic techniques and contaminated equipment (16,41).
Cantwell and Kasmire (42) referred a significant increase in the number of bacteria during the cutting process, automatic filling, and packaging lines of lettuce. It seems that a clean product can be reinfected after passing through stages in which there is the possibility of remaining vegetables, such as cutting, filling in equipment, and packaging. Several studies conducted on the production of these items in the workshops (43)(44)(45) showed that the surfaces of cutting tools, peeling machines, centrifuge systems, and the sorting room-air are the most polluted parts and that secondary contamination of products can occur anywhere in the production and contribution line.
None of the heat or freezing process is used in the production of fresh ready-to-eat vegetables for removing microorganisms, and the common processes for preparing vegetables with minimal process include sorting, cleaning, disinfecting, peeling, cutting, drying, packing, cold storage, and transport to distribution centres, depending on the products (18,46). In most cases, failure of the cold chain management reduces the shelf-life of the products (46).
However, disinfection with chlorine is the main method for reducing pathogenic organisms (13) but several factors, including biofilm formation by bacteria, internalization of pathogens within plant tissue, and the hydrophobicity of plant surfaces, decrease its effectiveness (47). There is a need to find alternative methods for preserving fresh-cut vegetables in order to improve the efficacy of washing treatments. Alternatives or modified methods have been proposed, including irradiation (48,49), ozone (50,51), bacteriophages, antagonistic bacteria (52,53), and essential oils (54,55). However, none has yet gained widespread acceptance by the industry. For this reason, there is a need to develop alternative methods and, subsequently, the need to develop markers to measure the efficacy of these alternatives.

Conclusions
Although the number of samples studied was small for some items due to sampling limitations, we believe this project provides a general overview of the microbiological quality of MPV and sprouts commercialized in Tehran, Iran. Due to high values of contamination found in MPV and sprout samples, it cannot be concluded that the fresh products analyzed have appropriate hygienic quality. MPV are subjected to various conditions during growth, harvest, preparation, packaging, and distribution that could cause increased contamination. These products are only treated by disinfection, which does not assure complete removal of microorganisms. Hence, these results suggest that measures, including good agricultural practices (GAP), good manufacturing practices (GMP), and Hazard Analysis and Critical Control Points (HACCP), should be implemented to reduce the risk of microbial contamination from farm-to-fork and to assure safe products.